karyology study on bleak (alburnus alburnus) from the south caspian sea region

the chromosomal spread and karyotype of bleak (alburnus alburnus) from anzali lagoon were identified using tissue squashing techniques with injection of 0.5ml/100g body weight of 0.01% colchicines solution in fish fingerlings. kidney and gill tissues were then extracted and chopped in kcl 0.045m for 20 min and fixed in carnoy solution in 3 stages. the chromosomal spreads were stained in 20% gimsa for 30 min. from 347 chromosomal spread counts, the results showed diploid chromosome number of this species 2n=50. karyotype composed of 7 metacentric, 13 submetacentric and 5 acrocentric or subtelocentric choromosome pairs, and the number of chromosome arms (nf) was determined as nf=90.